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Fig. 5. Smurf1 and Smurf2 differentially regulate mesodermal markers at gastrula stages. A: Overexpression of Smurf2 in either DMZ or VMZ disrupts expression of the pan-mesodermal marker Brachyury (XBra). Dorsal expression of Smurf2 slightly reduces the dorsal mesoderm marker chordin, whereas ventral expression of Smurf2 inhibits the ventral-lateral mesodermal marker wnt8. B: Unlike Smurf2, Smurf1 does not block, but instead mildly expands, the expression of XBra or chordin, though like Smurf2, it also inhibits wnt8 expression. Co-expression of Smurf1 and 2 leads to reduction of all three mesodermal markers. The numbers indicate the embryos displaying the shown changes in marker expression. |
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Fig. 10. Smurf1 and Smurf2 differentially regulate mesodermal marker expression. A: Knockdown of Smurf2 in the marginal zone region caused expansion of the pan-mesodermal marker XBra towards the ectodermal region. Dorsal knockdown of Smurf2 reduced expression of chordin,whereas ventral knockdown of Smurf2 led to expansion of wnt8 into the ectodermal domain. B: Unlike Smurf2 knockdown, Smurf1 knockdown reduced the expression of both XBra and chordin, and double knockdown of both Smurfs resulted in more efficient reduction of these markers. The numbers indicate the embryos displaying the shown changes of marker expression. |
