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Fig. 1 (left). Overexpression of tcf1 induces a dorsalized phe- notype in Xenopus embryos. (A) Xenopus tropicalis embryos overexpressing tcf1 display premature mesodermal involution. Stage 10.5 embryos, marginally injected in both blastomeres at the two-cell stage with 180 pg of tcf1 RNA, show premature mesodermal involution around the entire marginal zone (81%, n=62; red arrowhead, ventral). (B) Overexpression of tcf1 induces dorsalization. Expression of chordin (83%, n=58) and goosecoid (83%, n=59) has expanded all around the blastophore in Xenopus laevis embryos injected with tcf1 RNA, while expression of wnt8 (73%, n=64) and cdx1 (89%, n=55) is lost. |
