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Fig. 7. dnBMP1 does not dorsalise UV-irradiated gastrulae. One nanogram of dnbmp1 mRNA was injected into a single blastomere at the four cell stage, either randomly (UV-irradiated embryos) or into a single ventral blastomere. Whole mount in situ hybridisation was performed on midgastrulae with probes for chordin (A-D), xmyf5 (E-H) and xwnt8 (I-L). (A) Control gastrulae showing localisation of chordin mRNA to the dorsal marginal zone. (B) dnbmp1 injected gastrulae showing increased expression of chordin mRNA in the dorsal marginal zone. (C) Control UV-irradiated gastrulae showing absence of chordin expression. (D) UV-irradiated gastrulae injected with dnbmp1 showing absence of chordin expression. (E) Control gastrulae showing localisation of xmyf5 mRNA to the dorsolateral marginal zone. (F) dnbmp1 injected gastrulae showing expansion of chordin expression domain into the ventral marginal zone (arrows). (G) Control UV-irradiated gastrulae showing absence of xmyf5 expression. (H) UV irradiated gastrulae injected with dnbmp1 showing absence of xmyf5 expression. (I) Control gastrulae showing localisation of xwnt8 mRNA to the ventral and lateral marginal zone. (J) dnbmp1 injected gastrulae showing reduction in xwnt8 expression (arrowheads). (K) Control UV-irradiated gastrulae showing expansion of xwnt8 expression into dorsal marginal zone. (L) UV-irradiated gastrulae injected with dnbmp1 showing continued
expression of xwnt8 throughout the marginal zone. |
