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Fig. 3. dnXBMP1 and dnXld dorsalise Xenopus embryos. (A) Normal stage 40 embryos injected with 1.5 ng of dnbmp1 mRNA into a single ventral blastomere at the four cell stage. Many embryos injected with this mRNA are normal. (B) Partial secondary axes in stage 40 embryos injected with 1.5 ng of dnbmp1 mRNA into a single ventral blastomere at the four cell stage. (C and D) Complete secondary axes in stage 40 embryos injected with 1.5 ng of dnbmp1 mRNA into a single ventral blastomere at the four cell stage. (E) Section through control embryo showing internal organisation of mesodermal tissues (no notochord) and neural tube (nt). (F) Section through embryo in D showing duplicated secondary axis includes neural tube, notochord and somites. (G±L) Whole mount in situ hybridisation analysis of midgastrulae (stage 11.5-12) for chordin (G and J), xmyf5 (H and K) and xwnt8 (I and L). (G) Uninjected embryos showing dorsal expression of chordin (arrows). (H) Uninjected embryos showing dorsolateral expression of xmyf5. (I) Uninjected embryos showing ventral and lateral expression of xwnt8. (J) dnbmp1 injected embryos showing ventral expression (arrowheads) of chordin. (K) dnbmp1 injected embryos showing ventral expression of xmyf5 (arrowheads). (L) dnbmp1 injected embryos showing reduced expression xwnt8. Embryos in (G-L) were injected with 1.5 ng of dnbmp1 mRNA into a single ventral blastomere at the four cell stage. |
