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Figure 4. Overexpression of PIAS disrupts early mesodermal formation. In situ hybridization was performed to assess mesodermal formation in embryos injected with PIAS RNAs. While all four PIAS genes inhibited expression of Chordin, XBra, and MyoD, they displayed different efficiency in doing so, with PIAS3 having weak XBra inhibitory activities. Unlike PIAS1, 2, and 4, PIAS3 did not block expression of Wnt8 efficiently. The doses of the RNAs used in this experiment were: 1 ng PIAS1, 0.5 ng PIAS2, 2 ng PIAS3, and 1 ng PIAS4.Download figure to PowerPoint |