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Figure 1. Expression of Xwnt8 during gastrula and early neurula stages. Embryos were assayed for expression of Xwnt8 by whole-mount in situ hybridization. In each panel, a single embryo is shown. A: Stage 11 embryo, lateral view with dorsal to the left, and a sagittal section of the embryo. Xwnt8 expression is detected in the ventral and lateral marginal zone mesoderm. B: Stage 11.5 embryo, lateral view with dorsal to the left, and a lateral to lateral section. Expression can be found close to the blastopore and in involuted mesoderm. C: Stage 12 embryo, lateral view with dorsal to the left, and a posterior view. Expression of Xwnt8 can be found in presumptive paraxial mesoderm and expression close to the blastopore is further restricted to dorsolateral positions. D: Stage 13 embryo, lateral view with anterior to the left, a posterior view of the embryo, and two transverse sections. In the section on the right top of the panel, Xwnt8 expression in presumptive hindbrain is shown, this corresponds to the anterior-most expression in the lateral view. Expression in mesoderm close to the closing blastopore is shown in the bottom right section of the panel and corresponds to expression shown in posterior view. E: Stage 17 embryo, lateral view with anterior to the left, posterior view, and a transverse section. The anterior ectodermal expression domain, the paraxial expression, and the dorsolateral expression in the mesoderm remain, while a lateral expression domain appears in the ectoderm. In the dorsal-to-ventral section, and in an enlargement on the bottom right of the panel, initiation of Xwnt8 expression in the neural tube can be found. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hoxc6
|
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laevis
|
NF stage 13
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ectoderm
,
mesoderm
,
circumblastoporal collar
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hoxc6
|
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laevis
|
NF stage 11.5
to
NF stage 12
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marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
non-involuting marginal zone
|
hoxc6
|
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laevis
|
NF stage 13
|
ectoderm
,
mesoderm
,
circumblastoporal collar
|
hoxc6
|
|
laevis
|
NF stage 11.5
to
NF stage 12
|
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
non-involuting marginal zone
|
hoxd1
|
|
laevis
|
NF stage 11
to
NF stage 11.5
|
mesoderm
,
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
[+]
|
hoxd1
|
|
laevis
|
NF stage 12
|
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
circumblastoporal collar
|
hoxd1
|
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laevis
|
NF stage 13
|
hindbrain
,
circumblastoporal collar
,
involuted dorsal mesoderm
,
neural plate
,
paraxial mesoderm
|
hoxd1
|
|
laevis
|
NF stage 11
to
NF stage 11.5
|
mesoderm
,
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
[+]
|
hoxd1
|
|
laevis
|
NF stage 12
|
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
circumblastoporal collar
|
hoxd1
|
|
laevis
|
NF stage 13
|
hindbrain
,
circumblastoporal collar
,
involuted dorsal mesoderm
,
neural plate
,
paraxial mesoderm
|
hoxb4
|
|
laevis
|
NF stage 11.5
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mesoderm
,
dorso-lateral marginal zone
|
hoxb4
|
|
laevis
|
NF stage 12
|
ectoderm
,
mesoderm
,
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
[+]
|
hoxb4
|
|
laevis
|
NF stage 13
|
mesoderm
,
circumblastoporal collar
,
involuted dorsal mesoderm
,
paraxial mesoderm
|
hoxb4
|
|
laevis
|
NF stage 11.5
|
mesoderm
,
dorso-lateral marginal zone
|
hoxb4
|
|
laevis
|
NF stage 12
|
ectoderm
,
mesoderm
,
marginal zone
,
ventral marginal zone
,
ventro-lateral marginal zone
,
[+]
|
hoxb4
|
|
laevis
|
NF stage 13
|
mesoderm
,
circumblastoporal collar
,
involuted dorsal mesoderm
,
paraxial mesoderm
|
|
|
Figure 2. Expression of Xwnt8, Hoxd1, Hoxb4, and Hoxc6 during gastrulation. A-D: Embryos were analyzed by whole-mount in situ hybridization for expression of Xwnt8 (A), Hoxd1 (B), Hoxb4 (C), and Hoxc6 (D). Embryos are shown, going from left to right through the panels, at stage 11, stage 11.5, stage 12 (vegetal views with dorsal up), and at stage 13 (vegetal views with dorsal up). Xwnt8 expression overlaps with the expression of Hoxd1 in the ventrolateral mesoderm during early gastrulation. At stage 12, the posterior most expression of Xwnt8 becomes restricted to dorsolateral marginal zone, overlapping with the expression domain of Hoxd1. When gastrulation is nearly completed, an overlap in expression of Xwnt8 and Hoxd1 can be observed in presumptive hindbrain, and paraxial mesoderm. Hoxb4 and Xwnt8 show an overlap in their expression patterns during stage 11.5, at stage 12 ectodermal expression of Hoxb4 is initiated in overlapping the dorsolateral Xwnt8 expression domain. During late gastrulation, an overlap in expression of Hoxb4 and Xwnt8 is observed in paraxial mesoderm. Expression of Hoxc6, on the other hand, is initiated after the retraction of the Xwnt8 expression to the dorsolateral domains; therefore, an overlap in expression is only observed there. This overlap is still visible at the end of gastrulation. Likewise for Hoxd1 and Hoxb4, the first ectodermal expression is found in the ectoderm overlying the posterior dorsolateral domains of XWnt8 expression. |