|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
frzb2
|
|
laevis
|
NF stage 10.5
|
dorsal marginal zone
,
prechordal plate
,
upper blastopore lip
,
deep
,
endomesoderm
|
frzb2
|
|
laevis
|
NF stage 13
|
prechordal plate
,
blastocoel roof
,
endomesoderm
,
anterior
|
frzb2
|
|
laevis
|
NF stage 17
to
NF stage 18
|
prechordal plate
,
dorsal
,
endomesoderm
,
anterior
|
wnt5a
|
|
laevis
|
NF stage 11.5
|
circumblastoporal collar
,
involuted dorsal mesoderm
,
involuted ventral mesoderm
,
endomesoderm
|
wnt5a
|
|
laevis
|
NF stage 18
|
ectoderm
,
mesoderm
,
circumblastoporal collar
,
posterior
,
non-neural ectoderm
|
|
|
Fig. 5. Comparison of the expression domain of crescent with those of Xwnt11 and Xwnt5a at early gastrula to mid neurula stages. (A) Dorsal view of early gastrula (animal pole is upwards). Expression domains of crescent (cyan) and Xwnt11 (purple) are situated close to each other at this stage. (B) An early-gastrula-stage embryo was bisected sagittally into halves, which were then hybridized for crescent (left panel) or Xwnt11 (right panel; flipped horizontally for comparison). The expression domains of crescent and Xwnt11 do not overlap but are adjacent. Dotted lines indicate the dorsal border of crescent expression. (C,D) Hemisections of early-neurula- (C) or mid-neurula-stage (D) embryos. The expression domains of crescent (cyan) and Xwnt11 (purple) progressively separate into the anterior endomesoderm and the surface layer of the circumblastoporal tissue, respectively. Anterior is to the left. (E) and (F) Hemisections of late gastrula (E) or mid neurula (F). The expression domains of crescent (cyan) and Xwnt5a (purple, indicated by arrows) progressively separate into the anterior endomesoderm and the surface layer of the circumblastoporal tissue, respectively. Anterior is to the left. Arrowheads, blastopores. Photographs of the hemisections stained for Xwnt5a were flipped horizontally for comparison. |