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Fig. 2. Expression pattern of Coco mRNA during Xenopus development. (A) Whole-mount in situ hybridisation of Coco, Bmp4 and VegT mRNA in the egg. Expression of Coco was also compared to Vg1 by RT-PCR. (B) Coco is expressed strongly in the animal pole at the 2-cell, 8-cell stages and at stage 8. (C) Coco is strongly expressed maternally and then is downregulated at the post-gastrula/pre-neurula stages. In contrast Cerberus is first expressed at stage 9 and then downregulated after the onset of neurulation. (D,E) At gastrula stage Coco is detected at high levels in the ectoderm and marginal zones by (D) whole-mount in situ hybridisation and (E) as seen by RT-PCR at much lower levels in the vegetal pole. ODC was used as a loading control for the RT-PCR. * indicates the organizer. |
