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Figure 2. FGF signaling effects on endoderm and mesoderm. These embryos have been processed by in situ hybridization for expression of the indicated transcript above each column. Embryos were treated with DMSO or SU5402 from the 8- to 16-cell stage as indicated on the side. A,B: Lateral views; embryos were collected at the blastula stage, 7.5 hr post-fertilization. C-S: Gastrula stage. C-N: Blastoporal views. O,P: Lateral views with vegetal to the bottom. Q-S: Embryos were injected into one cell at the two-cell stage with the dominant-negative FGFR1 construct XFD (500 pg); the red staining is the lineage tracer n beta gal. T: Whole embryos were treated with either DMSO or SU5402, collected at the time indicated above the lanes, and analyzed by RT-PCR for expression of the indicated markers along the side. ODC was used as a loading control. |
