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Fig. 4. Apod mRNA shows a more widespread distribution than Xbra mRNA in the gastrula. In situ hybridisation was performed on whole-mount (A,B,E,F) or sectioned (C,D) embryos. The dorsal side is to the right in A-D, and the position of the dorsal lip is indicated with a solid arrow. Late stage gastrulae were also hybridised with probes for Apod mRNA as whole mounts (E,F) and show its presence in the posterior paraxial mesoderm and ventral blastopore. Apod mRNA is notably excluded from the notochord (indicated with an open arrow). (A,C) Apod probe, stage 10.5. (B,D) Xbra probe, stage 10.5. (E) Posterior view, dorsal side at top; Apod probe stage 13. (F) Dorsal view, anterior end at top; Apod probe, stage 13. |
