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Fig. S1. Immunodetection of endogenous Vangl2 protein.
(A, B) Basolateral localization (arrowhead) of Vangl2 (A) and b1-integrin (B) in embryonic ectoderm at stage 11. (C) Immunoblot of ectodermal cell lysates with anti- Vangl2 antibody reveals a 60 kDa band (arrowhead) corresponding to endogenous Vangl2, misexpressed Xenopus CFP-Vangl2 and mouse HA-VL2, as indicated.(D-G) Vangl2 immunostaining on cross-sections of st. 10- and st. 10.5 embryos. Dashed boxes in D and E correspond to the magnified images shown in D’ and E’. Vangl2 is detected at the basolateral membrane and in cytoplasmic vesicles near the apical cortex, and appears more abundant in the superficial cell layer. Scale bar in A is 20 μm. Both apical (arrow) and basolateral staining is detected in the constricting blastopore cells of st. 10.5 embryo. D-G, Scale bar is 20 μm. |
