| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| vangl2 | xenopus | epidermis [+] |
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Experiment details for vangl2
The roles of maternal Vangl2 and aPKC in Xenopus oocyte and embryo patterning.
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| Fig. 3. Vangl2 interacts with VAMP1. (A) Histological section of a stage 6 Xenopus oocyte co-immunostained for Vangl2 (VG) and VAMP1. (B,B′) Colocalization of Vangl2 (red) and VAMP1 (green) in the stage 6 oocyte cytoplasm. B′ shows a high magnification image (×100 oil objective with digital zoom) of the boxed area in B demonstrating the co-distribution of Vangl2 and VAMP1 (yellow). (C) Co-immunoprecipitation analysis of lysates from wild-type and Vangl2 mRNA (VG+; 500 pg)-injected stage 6 (St. VI) oocytes showing that endogenous Vangl2 protein (VG) interacts with endogenous VAMP1 protein (VAMP). Overexpressed Vangl2 reduces the total Vangl2 interaction with VAMP1. The expression level of VAMP1 in these samples is shown in the input lanes. (D) Co-immunoprecipitation analysis of an uninjected oocyte showing that endogenous VAMP1 protein interacts with endogenous Vangl2 protein. Mouse IgG serves as a negative control for non-specific binding of Xenopus oocyte lysates to the bead. (E) Co-immunostaining for Vangl2 (VG) and VAMP1 protein in histological sections of stage 45 tadpole skin. DAPI staining shows nuclei in the bilayered epidermis. Colocalization of VG and VAMP1 is most obvious on the basal side of the outer layer of the epithelium (chevrons, yellow staining in merge). |