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twist1xenopus   

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Experiment details for twist1

Wu CF et al. (2001) Assay

Tumorhead, a Xenopus gene product that inhibits neural differentiation through regulation of proliferation.

Gene Clone Species Stages Anatomy
twist1 xenopus NF stage 18 neural crest , neural tube

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  Fig. 3. Effect of TH overexpression on molecular markers. (A-H) Effect of TH on expression of early neural markers. (A) Control embryo at stage 15 showing normal in situ hybridization pattern of Xsox-2. (B) Xsox-2 expression is expanded on side of embryo that is overexpressing TH (arrow and brackets). (C) Control embryo at stage 16 showing normal in situ hybridization pattern of NCAM. (D) NCAM expression is expanded in TH overexpressing region (arrow and brackets). (E) Normal pattern of Zic-related-1 mRNA in stage 17 embryo. (F) Expanded region of Zic-related-1 expression in TH-injected embryo (arrow and brackets). (G) Normal pattern of Zic3 mRNA in stage 14 embryo. (H) Inhibition of Zic3 in region overexpressing TH (arrow). (I-L) Effect of TH overexpression of late neural markers: (I) control stage 18 embryo hybridized with NeuroD probe; (J) inhibition of NeuroD on side of embryo overexpressing TH (arrow); (K) control stage 18 embryo showing the pattern of expression of N-tubulin mRNA; (L) inhibition of N-tubulin on side of embryo overexpressing TH (arrow). (M-T) Effect of TH on neural crest and mesoderm markers: (M) control stage 18 embryo probed by in situ hybridization with the Xtwist probe showing normal pattern of twist mRNA; (N) inhibition of twist in region overexpressing TH (arrow); (O) control embryo showing the expression of Xslug mRNA; (P) inhibition of Xslug mRNA in region overexpressing TH (arrow); (Q) normal pattern of Xnot mRNA expression in stage12 embryo; (R) normal pattern of Xnot expression in embryo overexpressing TH (arrow); (S) normal pattern of chordin mRNA expression in stage 10.5 embryo; (T) normal pattern of chordin expression in area overexpressing TH (arrow).