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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
twist1	xenopus

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Experiment details for twist1

Vignal E et al. (2007) Assay

Expression of RhoB in the developing Xenopus laevis embryo.

Gene	Clone	Species	Stages	Anatomy
twist1.S		laevis	NF stage 16	neural crest

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Fig. 4. Spatial expression pattern of XrhoB during early neurula stages. (A–B) Stage 14. (A) Dorsal view. (B) Anterior view. (C–D) Transverse paraffin sections across the C and D lines shown in (A) and (B). In (C), the black arrowhead shows RhoB staining in posterior paraxial mesoderm and the white, in axial tissue. (E) XrhoB expression pattern at stage 16. Dorsal view. XrhoB is expressed in the developing central nervous system, both in the forebrain and the hindbrain and accumulates in specific rhombomeres. (F) Stage 16 embryos hybridized with XrhoB (purple) and Xkrox20 (red) antisense probes. Dorsal view. Krox20 is expressed in rhombomeres 3 and 5 (R3 and R5) and in the neural crest cells (nc) adjacent to R5. In the hindbrain, expression of XrhoB accumulates in R4, based on the expression domains of krox20, and in a rhombomere above R3 (white arrowhead) that may correspond to R1 or R2 based on distances between streaks. (G) Xtwist staining at stage 16. Dorsal view. Xtwist is expressed in neural crest cells at the lateral edges of the neural plate. (H) XrhoB expression at stage 16. Dorsal view. In the trunk region, XrhoB is expressed in the neural tube and the developing somites. (I) XrhoB staining at stage 22. Dorsal view. (J) Transverse paraffin section across the J line of the embryo shown in (I). XrhoB is expressed in the dorsal part of the neural tube and in cranial migrating neural crest cells. (K) Xtwist staining at stage 22. Dorsal view. (L) Transverse paraffin section across the L line of the embryo shown in (K). Abbreviations: anr, anterior neural ridge; for, forebrain; hb, hindbrain; mnc, migrating neural crest cells; nc, neural crest cells; no, notochord; nt, neural tube; R3, rhombomere 3; R4, rhombomere 4; R5, rhombomere 5; so, somites.

Gene	Clone	Species	Stages	Anatomy
twist1.S		laevis	NF stage 27	mandibular crest , hyoid crest , branchial crest

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Fig. 5. Spatial expression pattern of XrhoB at late neurula stages. (A–B) Stage 27. Lateral views. (B) Magnified view of the head of the embryo shown in A. (C) Xtwist staining at stage 27. (D) Horizontal and (E) vertical paraffin sections across the D and E lines of the embryo shown in (A). XrhoB is expressed in the anterior and posterior branchial, hyoid and mandibular crest segments. (F and G) Transverse paraffin sections across the F and G lines of the embryo shown in (A). The blue arrowhead in (F) shows the dorso-lateral part of the somite and the red arrowhead, the dorsal part of the neural tube. (H–J) Stage 34. (H–I) Lateral views. (I) Magnified view of the head of the embryo shown in H. (J) Dorsal view. Abbreviations: ab, anterior branchial crest segment; pb, posterior branchial crest segment; hc, hyoid crest segment; mc, mandibular crest segment; df, dorsal fin; le, lens; mhb, midbrain hindrain boundary; mnc, migrating neural crest cells; no, notochord; ov, otic vesicle nt, neural tube; so, somites; upm, unsegmented presomitic mesoderm.