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Fig. 3. Snail participates in the early specification of the neural crest. One blastomere of a two-cell stage embryo was injected with 700 pg of Xsnail-GR mRNA, treated with dexamethasone at stage 12.5, fixed at stage 19, and the expression of several genes analysed. The arrowheads indicate the injected side that contained FLDx (see Materials and Methods). Anterior is towards the left. (A-D) Neural crest markers. Notice the expansion of the markers on the injected side. (A) Snail expression. (B) Slug expression. (C) Twist expression. (D) FoxD3 expression. (E) Expression of the neural plate marker Sox2, is reduced on the injected side. The broken line indicates the dorsal midline and the brackets indicate the width of the neural plate. (F) Expression of the epidermal marker Cytokeratin 81A (dorsal view), is almost completely inhibited on the injected side. (G,H) Lateral views of the same embryo where the inhibition of Cytokeratin expression is better assessed. G corresponds to the injected side. |
