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Figure 6. xKazrin or xARVCF depletion perturbs the expression of neural crest cell markers. A: The indicated morpholinos (20 ng) were injected into the animal region of one dorsal blastomere (animal region) of four-cell embryos. At early neurula stages (stage 15), embryos were fixed and neural crest cells visualized using whole-mount in situ hybridization for two neural crest markers: Twist and FoxD3. B,C: Embryos were fixed at early tail bud stages, stages 20 and 22 (respectively B and C) and whole-mount in situ hybridization for twist was performed. Unseparated branchial neural crest cell streams are indicated by brackets. Asterisks (*) indicate the injected side. Br, Branchial neural crest cells; aBR, anterior branchial neural crest cells; pBR, posterior branchial neural crest cells; H, Hyoid neural crest cells; M, Mandibular neural crest cells.Download figure to PowerPoint |
