|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
tubb2b
|
|
tropicalis
|
NF stage 24
to
NF stage 28
|
brain
,
spinal cord
,
cranial ganglion
,
eye
,
neural tube
|
tubb2b
|
|
tropicalis
|
NF stage 42
|
brain
,
spinal cord
,
eye
,
head
,
neural tube
|
epha4
|
|
tropicalis
|
NF stage 24
|
brain
,
hindbrain
,
branchial crest
,
head region
,
neuroectoderm
,
[+]
|
epha4
|
|
tropicalis
|
NF stage 28
|
otic vesicle
,
brain
,
hindbrain
,
pharyngeal arch
,
branchial arch
,
[+]
|
ric8a
|
|
tropicalis
|
NF stage 22
|
brain
,
eye
,
posterior neural tube
,
cranial placode
,
anterior neural tube
,
[+]
|
ric8a
|
|
tropicalis
|
NF stage 25
|
brain
,
midbrain-hindbrain boundary
,
mandibular crest
,
hyoid crest
,
branchial crest
,
[+]
|
ric8a
|
|
tropicalis
|
NF stage 33 and 34
|
otic vesicle
,
brain
,
forebrain
,
hindbrain
,
midbrain-hindbrain boundary
,
[+]
|
ric8a
|
|
tropicalis
|
NF stage 42
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
midbrain-hindbrain boundary
,
[+]
|
ric8a
|
|
laevis
|
NF stage 22
|
brain
,
hindbrain
,
eye
,
posterior neural tube
,
neural tube
,
[+]
|
ric8a
|
|
laevis
|
NF stage 28
to
NF stage 32
|
brain
,
cranial ganglion
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
[+]
|
|
|
Fig.4. Comparative analysis of XtRIC-8 and control markers by whole mount in situ hybridization at later developmental stages of Xenopus tropicalis embryos. (A) The tissue specific expression pattern of ric-8 at different later stages of X. laevis and X. tropicalis embryonic development was analyzed by whole mount in situ hybridization. Probes are indicated in each panel at the left and stages are indicated at the top of each panel. XtRIC-8 (panels e–h) and XlRIC-8 (panels i–k) antisense probe reactions are shown. XtRIC-8 sense probe was used as negative control (panels a–d). Lateral views are shown for embryos at stage 22, 25, 28, 32 and 34, and dorsal views are shown for embryos at stage 22 and 42. For embryos at stages 25, 34 and 42 that were hybridized with the antisense XtRIC-8 probe, a higher magnification view from the head is also shown in the inset (panels f, g and h). (B) The tissue specific expression of different control markers were determined at different developmental stages of X. tropicalis embryos by whole mount in situ hybridization. N-tubulin, EphA4 and Twist were used as control markers. Probes are indicated at the left side of each row and stages at the bottom-left side of each panel. Lateral views for stages 24, 28 and dorsal views for stage 42. Red arrows indicate specific structures that were labeled as: nt (neural tube), mhb (mid/hindbrain), b (brain), fb (forebrain), opc (optic cup), oc (otic capsule), ba (branchial arches), pg (pineal gland), rl (rhombic lip) and ek (embryonic kidney). |