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Fig. 5. Xphb1 acts downstream of c-myc and upstream of twist, snail2 and foxD3. (A) Injection of c-myc MO (*) efficiently inhibited Xphb1 expression (90%, n=116). (B) Xenopus embryos injected with c-myc MO and analyzed by quantitative real-time PCR showed a reduction in relative Xphb1 expression (normalized to the level of ODC expression). **, P<0.005. (C) c-myc MO-mediated depletion of twist, snail2 and foxD3 was rescued by co-injection of Xphb1 mRNA (twist, **, P<0.005; snail2, *, P<0.05; foxD3, **, P<0.005), whereas Xphb2 had no effect. n, number of embryos analyzed. Error bars indicate standard error. |
