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Fig. 9. E2F1 transcriptional activity is regulated by Xphb1 and affects neural crest formation. (A) Luciferase reporter assay performed with Xenopus embryos injected with Gal4-DBD-E2F1, pFR trans-reporter plasmid (control) and different concentrations of Xphb1 mRNA. The transcriptional activity of E2F1 decreased when Xphb1 was overexpressed. (B) Injection (*) of human E2F1 DNA inhibited twist expression in a concentration-dependent manner. This was rescued by co-injection of Xphb1 mRNA. (C) Knockdown of Xphb1 by phb1 MO (3 ng) together with human E2F1 DNA injection (*) act synergistically in repressing twist expression. n, number of embryos analyzed. **, P<0.005; *, P<0.05. Error bars indicate standard error. |
