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twist1xenopus   

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Experiment details for twist1



Diversification of the expression patterns and developmental functions of the dishevelled gene family during chordate evolution.

Gene Clone Species Stages Anatomy
twist1 xenopus NF stage 18 neural crest , eye primordium , anterior neural fold , anterior branchial crest

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  Figure 5. Neural crest induction in Xenopus requires Dvl1 and Dvl2, but not Dvl3. A-A rdquo : Unilateral injection of MOs + GFP mRNA targeted to one dorsal lateral blastomere of a 16-cell embryo. Slug expression is lost on the injected side due to Dvl1 and Dvl2 morpholinos. B-B rdquo : Dvl1 and Dvl2 MOs eliminate expression of Twist. C,C': Graphs of combined morpholino data for Slug in situ hybridizations. Data shown are percent of embryos showing normal or abnormal expression of Slug or Twist. Uninjected Control N=16; Dvl-1MO 72 ng, N=26; Dvl-2MO 72 ng, N=10; Dvl-3MO 72 ng, N=12; Dvl-1MO + Dvl-2MNO 36 ng each, N=15; Dvl-1MMMO 72 ng, N=13; Dvl-1MO + Dvl-1MMMO 36 ng each, N=25; Dvl-2MO + Dvl-1MMMO 36 ng each, N=11. D,D': Graphs of combined data for Twist in situ hybridizations. Uninjected Control N=36; Dvl-1MO 72 ng N=84; Dvl-2MO 72 ng, N=28; Dvl-3MO 72 ng, N=20; Dvl-1MO + Dvl-2MNO 36 ng each, N=31; Dvl-1MMMO 72 ng, N=54; Dvl-1MO + Dvl-1MMMO 36ng each, N=18; Dvl-2MO + Dvl-1MMMO 36 ng each, N=24.