| |
Figure S4: WDR5 is localized near the base of cilia in the MCCs. Related to Figure
2.
(A) X. tropicalis expressing WDR5-GFP. WDR5 is localized apically in the MCCs in a
punctate pattern (white arrows) and in the membrane and nuclei of epithelial cells.
(B) X. tropicalis epidermal MCC expressing WDR5-GFP (green) and centrin-RFP (red)
shows that WDR5 localizes near the basal bodies.
(C) Immunofluorescence showing localization of WDR5 (green) and γ-tubulin (red) in a
MCC of X. tropicalis epidermis.
(D) Immunofluorescence showing localization of WDR5 (red), acetylated α-tubulin
(yellow) in MCCs of mouse trachea. WDR5 signal is lost after incubating the antibody
with the blocking peptide. |
