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tubb2bxenopus   

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Experiment details for tubb2b

Solini GE et al. (2020) Assay

Xenopus embryos show a compensatory response following perturbation of the Notch signaling pathway.

Gene Clone Species Stages Anatomy
tubb2b.L laevis NF stage 18 pre-chordal neural plate , neural groove , neural plate , anterior neural fold , Rohon-Beard neuron
tubb2b.L laevis NF stage 28 forebrain , midbrain , hindbrain , spinal cord , trigeminal nerve , [+]
tubb2b.L laevis NF stage 37 and 38 olfactory placode , forebrain , midbrain , hindbrain , spinal cord , [+]

  Fig. 1. tubb2b expression in response to Notch perturbation. Embryos unilaterally injected with 1.5 ​ng ICD or DBM show perturbed expression of tubb2b at neurula stages on the injected (left) side (A, B, G, H). The difference in expression between sides is less stark at the tailbud stage (C, D, I, J), and minimal difference between sides is apparent at the swimming tadpole stage (E, F, K–N). All embryos shown are left-side injected. Histological analysis of tubb2b expression in Notch perturbed embryos supports whole-mount findings (G-N). Abbreviations: l, lateral longitudinal stripe of primary neurogenesis; i/m, intermediate/medial longitudinal stripes of primary neurogenesis; asc, anterior spinal cord; e, eye; fb, forebrain; hb, hindbrain; mb, midbrain; sc, spinal cord; V, cranial nerve V; IX, cranial nerve IX; X, cranial nerve X. For each stage and each condition, N = >100 for whole mount embryos and >15 for histological analyses.

Gene Clone Species Stages Anatomy
tubb2b.L laevis NF stage 18 eye primordium , neural plate , anterior neural fold , Rohon-Beard neuron
tubb2b.L laevis NF stage 28 forebrain , midbrain , hindbrain , spinal cord , eye
tubb2b.L laevis NF stage 37 and 38 brain , spinal cord , eye

  Fig. 3. tubb2b expression in bilaterally perturbed embryos. Embryos were bilaterally injected at the two-cell stage with either ICD (A-C, G-I) or DBM (D-F, J-L) mRNA and reared to desired stages. ICD bilaterally injected embryos demonstrate minimal tubb2b expression at the neurula stage (A,G, arrowhead) and show morphological defects by the tailbud stage (B,H, arrowhead). DBM bilaterally injected embryos show ectopic tubb2b expression at the neurula stage (D,J, arrowhead), but appear to compensate in a gradual manner over time (E-F, K-L). GFP bilaterally injected embryos and wild type remain unperturbed (M-R). Abbreviations: i/m, intermediate/medial longitudinal stripes of primary neurogenesis; fb, forebrain; mb, midbrain; hb, hindbrain; e, eye; sc, spinal cord. For each stage, N = >50 for ICD and GFP, >10 for DBM. Embryos were obtained from a minimum of three different clutches.