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Figure 4: Cross-section of stage 30 X. laevis tadpoles and staining in the neural tissue, 20X. (A) Schematics indicating the relative positions (forebrain, midbrain, hindbrain, and spinal cord) of section planes on Xenopus tadpoles. (B) Corresponding cross-sections stained with anti-Sox3 (Red), anti-acetylated-tubulin (Green), and DAPI (Blue), showing the relative locations of neural stem cell pools as well as neurofilaments within the neural tube. (C) Corresponding cross-sections stained with anti-MyT1 (Red) and DAPI (Blue), showing the relative locations of differentiated primary neurons within the neural tube. Scale bar = 20 m. Xenopus illustration Natalya Zahn (2022) |
