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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
tubb2b	xenopus

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Experiment details for tubb2b

Bao J et al. (2000) Assay

Regulation of neurogenesis by interactions between HEN1 and neuronal LMO proteins.

Gene	Clone	Species	Stages	Anatomy
tubb2b.L		laevis	NF stage 14	brain , spinal cord , neuroectoderm , pre-chordal neural plate , chordal neural plate , [+] central nervous system , neural groove , neural plate , cranial placode , Rohon-Beard neuron , neuron , neural plate border , motor neuron , interneuron , neurogenic placode
tubb2b.L		laevis	NF stage 25	brain , forebrain , midbrain , hindbrain , spinal cord , [+] Rohon-Beard neuron , neuron , motor neuron , interneuron , neurogenic placode

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Fig. 4. Comparison of the expression patterns of XHEN1, XLMO-3, XNGNR- 1 and N-tubulin. (A-J) Stage 14, 25 and 35 Xenopus embryos were hybridized with probes for XLMO-3, XHEN1, X-NGNR-1 and N-tubulin. Embryos are shown in a dorsal (stage 14), dorsal-lateral (stage 25) or lateral (stage 35) view, with anterior to the left. At stage 14, XLMO-3 and XHEN1 are expressed only in two domains which will become interneurons (I) and motoneurons (M) of spinal cord, while X-NGNR-1 and N-tubulin are expressed also in the domains which will form sensory neurons (S) besides of these two medial domains. At stage 25, the expression patterns for all four probes are very similar. At stage 35, XHEN1 is only expressed in the brain region. (K,L) Transverse sections of Xenopus embryos hybridized with probes for XHEN1 and XLMO-3 at stage 20. In the neural tube, both of XHEN1 and XLMO-3 are located in the ventral part of spinal cord which is formed by interneurons and motoneurons.

Gene	Clone	Species	Stages	Anatomy
tubb2b.L		laevis	NF stage 14 to NF stage 25	neural plate , neuron , neural tube
tubb2b.L		laevis	NF stage 25	brain , forebrain , midbrain , hindbrain , spinal cord , [+] otic placode , trigeminal placode , optic vesicle , Rohon-Beard neuron , neuron , motor neuron , interneuron , neurogenic placode

Fig. 7. Ectopic neurogenesis induced by XHEN1 and/or XLMO-3 mRNAs. (A) Ectopic neurogenesis was visualized at stage 14 and stage 25 embryos by whole mount in situ hybridization with an N-tubulin probe. Embryos were injected on one side (left side at the bottom) with XLMO-3, XHEN1, XHEN1/XLMO-3 or X-NGNR-1. All embryos are shown with anterior to the left. Only the injected half of stage 25 embryos is shown. (B) Downregulation of neurogenesis by overexpression of XHEN1-WPRW. Ectopic neurogenesis was visualized at stage 14 by N-tubulin staining. One cell of the 2-cell stage embryos were injected with 500 pg of mRNA encoding for XHEN1-WRPW. The injected half (bottom half) showed little N-tubulin expression (right panel) when compared to the uninjected control (left panel). (C) Temporal Induction of N-CAM and N-tubulin in animal caps by over-expression of XHEN1 and XLMO-3. EF-1a is a ubiquitously expressed mRNA and serves as a control for RT-PCR and loading.