| |
Fig. 4. Comparison of the distribution of PACAP mRNA with that
of neuronal-specific class II b-tubulin (N-tubulin) in the neural tube of
Xenopus embryos. A,B and C,D illustrate the localization of N-tubulin
and PACAP mRNA in the neural tube of stage 28 embryos, respectively.
Whole-mount in situ hybridization revealed a distribution of
N-tubulin mRNA (arrow in A) similar to that of PACAP mRNA (arrow
in C) in the spinal cord (SC). Inside the spinal cord, N-tubulin mRNA
hybridization signals (arrows in B) were present mainly in the Rohon-
Beard neurons (RB) and motoneurons (MN). N-tubulin mRNA was
also detected in the interneurons between Rohon-Beard and motoneurons
(arrowhead in B). Conversely, throughout the spinal cord,
PACAP mRNA signals were found only in the dorsolateral part of the
spinal cord, corresponding to the positions of Rohon-Beard neurons
(arrow in D). Dashed lines in B,D outline the approximate boundary
of the neural tube. N, notochord. Scale bar 5 300 mm in C (applies to
A,C), 100 mm in D (applies to B,D). |
