|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
dll1
|
|
laevis
|
NF stage 16
|
neural plate
|
sox2
|
|
laevis
|
NF stage 14
|
neural plate
|
otx2
|
|
laevis
|
NF stage 14
|
pre-chordal neural plate
,
anterior
|
snai2
|
|
laevis
|
NF stage 16
|
mandibular crest
,
hyoid crest
,
branchial crest
,
cranial neural crest
|
nkx1-2
|
|
laevis
|
NF stage 12
|
ectoderm
|
nkx1-2
|
|
laevis
|
NF stage 14
|
chordal neural plate
,
neural crest
,
neural plate
,
cranial neural crest
,
neural plate border
|
nkx1-2
|
|
laevis
|
NF stage 18
|
hyoid crest
,
branchial crest
,
chordal neural plate
,
neural plate
,
cranial neural crest
,
[+]
|
nkx1-2
|
|
laevis
|
NF stage 20
|
posterior neural tube
,
cranial neural crest
,
neural tube
|
nkx1-2
|
|
laevis
|
NF stage 25
|
posterior neural tube
,
posterior
,
neural tube
|
nkx1-2
|
|
laevis
|
NF stage 29 and 30
|
midbrain
,
spinal cord
,
posterior
|
nkx1-2
|
|
laevis
|
NF stage 16
|
hyoid crest
,
branchial crest
,
cranial neural crest
,
chordal neural plate border
,
neural plate border
|
ncam1
|
|
laevis
|
NF stage 16
|
neuroectoderm
|
|
|
Fig. 2. Temporal and spatial expression of Nbx during Xenopus development. (A) Temporal expression patterns of Nbx. RNA was extracted from embryos
at the indicated stage of development. Nbx mRNA expression levels were measured by RT-PCR. The ubiquitous marker histone H4 served as a control. (B�I)
Spatial expression patterns of Nbx. Nbx expression was analyzed by whole-mount in situ hybridization. (B) Stage 12. Black arrowheads in (B) indicate outer
stripes, and white arrowheads indicate inner stripes of Nbx expression. (C) Stage 14. (D) Stage 18. (E) Stage 20. (F) Stage 25. (G) Stage 30. Arrow in (G)
indicates new expression of Nbx in mesencephalon, and a transverse section of this region is shown in (I). (H) A transverse section showing the outer stripes
at stage 14. (I) A transverse section of Nbx-expressing region in mesencephalon at Stage 30. (J�T) Comparison between recognized markers and Nbx
expression. Whole-mount double in situ hybridizations were performed with a digoxigenin-labeled Nbx probe and a fluorescein-labeled probe for another
marker. (J) Nbx (purple) and Otx2 (blue) at stage 14. (K) Nbx (purple) and Sox2 (blue) at stage 14. (L) Nbx (purple) and N-CAM (blue) at stage 16. (M, N)
Nbx (purple) and Slug (blue) at stage 16. The 1 in (M) indicates the region where only Slug was expressed; the 2 indicates where Slug and Nbx were
coexpressed (a transverse section of this region is shown in N); and the 3 indicates where only Nbx was expressed. (O) Nbx (purple) and Delta-1 (blue) at
stage 16. (P) Nbx (purple) and -tubulin (blue) at stage 16. (Q) A schematic representation of the expression of Nbx and -tubulin. (R) An enlarged view
of the outer stripe region at stage 16. Nbx (purple) and -tubulin (blue). (S, T) Nbx (purple) and - tubulin at stage 25. The transverse section of posterior
region is shown in (T). |