|
Gene |
Clone |
Species |
Stages |
Anatomy |
tubb2b.S
|
|
laevis
|
NF stage 9
|
ectoderm
|
tubb2b.S
|
|
laevis
|
NF stage 11.5
to
NF stage 12
|
ectoderm
,
neuroectoderm
,
non-neural ectoderm
|
tubb2b.S
|
|
laevis
|
NF stage 13
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
tubb2b.S
|
|
laevis
|
NF stage 17
|
neural fold
|
tubb2b.S
|
|
laevis
|
NF stage 19
|
brain
,
spinal cord
,
cement gland primordium
,
eye primordium
,
neural tube
,
[+]
|
tubb2b.S
|
|
laevis
|
NF stage 37 and 38
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
spinal cord
,
[+]
|
tubb2b.S
|
|
laevis
|
NF stage 43
|
brain
,
spinal cord
,
optic nerve
,
cephalic nerve
,
cephalic nerve
|
|
|
Fig. 1. Localization of a neural-specific mRNA by whole-mount in situ hybridization. (A) Late blastula (stage 9; Nieuwkoop and Faber. 1967). The
signal is found in the dorsal (O) and the ventral (V) ectoderm. AP animal pole, VP vegetal pole. (8) Mid-gastrula (stage 11 1/2, large yolk plug; lateral view)
The signal can be identified on the dorsal side (O) and also in the ventral (V, presumptive epidermis) and lateral parts (YP yolk plug). (C) Late gastrula (stage
12; dorsal view). The presumptive neural plate is intensively stained (left side: cranial, right side: caudal). (DJ Early neurula (stage 13; lateral view). The
signal is now found mainly in the neural plate. (E) Late neural fold stage (stage 17. dorsal view). Only the presumptive brain area and the presumptive
spinal cord are stained. (F, G) Initial neural tube stage (stage 19. F dorsal view. G view on the furure head area). The signal is restricted to the presumptive
brain and spinal cord (SP) zone. A/so the presumptive cement gland (CE) /s stained. (H, J) Larval stage (stage 37; H lareral view, J dorsal view). The
distribution of the transcript can be found In the brain and the spinal cord. Note that also the optic nerve (OP) is stained. (KJ Advanced larva (stage 43,
dorsal view). The staining is detected in the brain. the spinal cord and a/so clearly in the optic nerve (arrow). |
|
|
|
Fig. 2 Cross section of an early neurula (stage 14, caudal part). It was
incubated with antisense 35S-RNA of the clone 24-10 (f3-tubulin). The signal
is observed by Epi-Polarization(Grunz. 1990c) in rhe inner layers of the
medullary plate only. e epithelial layer of the neuroectoderm,s sensorial
layer of the neuroectoderm, a archenteron. |