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FIG. 6. Overexpression analysis of XBrn3d. Embryos were injected at the two-cell stage with mRNA for XBrn3d, then analyzed at stage 16 7 or stage 28 2 by whole-m ount in situ hybridization. ﰁ-Gal was coinjected to m ark the injected side of the em bryo. (A) Overexpression of XBrn3d causes loss of N-tubulin expression on the injected side (area under left arrowhead). (B) Expression of XamL, which marks Rohoneard cells, was also reduced at stage 17, indicating a loss of primary sensory neurons (area under left arrowhead). (C, D) At tailbud stages, ectopic N -tubulin positive neurons are seen on the injected side. (E, F) In situ hybridization with the sensory ganglia marker Xhox11L2 at stage 32. Ectopic neurons are seen on the injected side of the embryo, indicating that at least some of these ectopic neurons are sensory in nature. Staining in cranial ganglia is also disrupted. The sky blue staining in E and F is nonspecific background. |