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FIG. 1 RA treatment specifically blocks myocardial differentiation in Xenopus embryos. Specific marker sequences have been detected in control (top) and experimental embryos using whole- mount in situ hybridization. All embryos were assayed at stage 34. (A) Detection of the myocardial-specific transcript XTnIc. (B)Detection of the a-cardiac actin sequence which is expressed in both cardiac and somitic muscle in the early Xenopus embryo. (C) Detection of neural specific b-tubulin type II transcripts. |
