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Fig. 5. Rescue from hnRNP K MO by co-injection of hnRNP K RNA. Two-cell embryos were unilaterally injected with hnRNP K MO1 plus 100 pg of hnRNP K RNA, then processed for whole-mount immunostaining for hnRNP K (A1,A2), neuronal β-tubulin (B1,B2) or NF-M (C1,C2). Images are stacks of five confocal microscopic optical sections taken from the uninjected (A1,B1,C1) and injected (A2,B2,C2) sides of the same animal, parasagitally through the spinal cord. SC, spinal cord; MA, motor axons; SN, cellular nuclei of somitic myotomes. Scale bars in A2,B2,C2 also apply to A1,B1,C1, respectively. |
