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tubb2bxenopus   

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Experiment details for tubb2b

Maldonado-Agurto R et al. (2011) Assay

Cloning and spatiotemporal expression of RIC-8 in Xenopus embryogenesis.

Gene Clone Species Stages Anatomy
tubb2b tropicalis NF stage 24 to NF stage 28 brain , spinal cord , cranial ganglion , eye , neural tube
tubb2b tropicalis NF stage 42 brain , spinal cord , eye , head , neural tube

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  Fig.4. Comparative analysis of XtRIC-8 and control markers by whole mount in situ hybridization at later developmental stages of Xenopus tropicalis embryos. (A) The tissue specific expression pattern of ric-8 at different later stages of X. laevis and X. tropicalis embryonic development was analyzed by whole mount in situ hybridization. Probes are indicated in each panel at the left and stages are indicated at the top of each panel. XtRIC-8 (panels e–h) and XlRIC-8 (panels i–k) antisense probe reactions are shown. XtRIC-8 sense probe was used as negative control (panels a–d). Lateral views are shown for embryos at stage 22, 25, 28, 32 and 34, and dorsal views are shown for embryos at stage 22 and 42. For embryos at stages 25, 34 and 42 that were hybridized with the antisense XtRIC-8 probe, a higher magnification view from the head is also shown in the inset (panels f, g and h). (B) The tissue specific expression of different control markers were determined at different developmental stages of X. tropicalis embryos by whole mount in situ hybridization. N-tubulin, EphA4 and Twist were used as control markers. Probes are indicated at the left side of each row and stages at the bottom-left side of each panel. Lateral views for stages 24, 28 and dorsal views for stage 42. Red arrows indicate specific structures that were labeled as: nt (neural tube), mhb (mid/hindbrain), b (brain), fb (forebrain), opc (optic cup), oc (otic capsule), ba (branchial arches), pg (pineal gland), rl (rhombic lip) and ek (embryonic kidney).

Gene Clone Species Stages Anatomy
tubb2b tropicalis NF stage 35 and 36 brain , hindbrain , spinal cord , cranial ganglion , eye , [+]
tubb2b tropicalis NF stage 42 otic vesicle , brain , hindbrain , eye , neural tube

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  Fig.5. Comparative cross-section in situ hybridization analysis of XtRIC-8, N-tubulin and EphA4 in two developmental stages of X. tropicalis embryos. The tissue specific expression of XtRIC-8, the neuronal marker N-tubulin and the neural crest marker EphA4, were determined at stage 35 and 42 of X. tropicalis embryos by whole mount in situ hybridization. Embryos were coronally sectioned every 16–20 μm. The black lines in the upper panels indicate the position of the visualized sections. The probes are indicated at the top of the figure and the embryo stage at the bottom-left of each panel. Lateral views are shown for stage 35 embryos. The bottom panel shows a representation of the cross section of a stage 42 embryo. Red arrows indicate specific structures that were labeled as: nt (neural tube), l (lens), oc (otic capsule), ov (otic vesicle), and sm (somites).