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Fig. 1. Virtually every tissue is affected during spontaneous metamorphic remodeling of the duodenum. Cross sections of the duodenum from (A–C) wild-type prometamorphic tadpoles NF57; (D–F), metamorphic climax NF61; (G–I), and the end of metamorphosis NF66. (C, F, and I) Cross sections of the duodenum from tadpoles transgenic for IFABP-GFP. The GFP antibody reaction is green; smooth muscle actin antibody is red. (A, D, and G) Hematoxylin and eosin. (B, E and H) Immunoreactivity against endogenous intestinal fatty acid binding protein (IFABP; blue), muscle-specific smooth muscle actin (red), enteric neuron-specific neural beta-tubulin (green); and a nuclear counter-stain (dapi; white) is shown for half of each section. t = typhlosole, c = circular muscle, l = longitudinal muscle. Scale bar in C denotes 0.2 mm length. |
