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Fig. 1. The proneural activities of Atonal-related proteins. (A-D) Whole-mount in situ hybridisation with an N-tubulin probe to visualise neurogenesis in Xenopus embryos at stage 15. (A) Uninjected embryos. (B) 500 pg Ngn1 mRNA. (C) 500 pg Ato mRNA. (D) 500 pg Math1 mRNA. (E) Part of a wild-type fly wing showing no sensory bristles along the AP axis. (F) A uasMath1/+; dppGal4/+ wing. (G) A uasngn1/+; dppGal4/+ wing. (H) Quantitative analysis of the number of ectopic bristles per fly induced by expression of MATH1 or NGN1 using dppGal4 driver, `n' is the number of flies counted. (I-K) Third instar larval (L3) wing discs stained with anti-β-GAL (green) and proneural antibodies (red). (I) An A101/TM6 wing disc. (J) A uasato,dppGal4/A101 wing disc, anti-ATO (red) and anti-β-GAL (green). (K) A uasngn1, dppGal4/A101 wing disc, anti-NGN1 (red), anti-β-GAL (green). (L-N) L3 wing discs stained with anti-ASE. (L) A wild-type fly (CS) wing disc. (M) A uasato,dppGal4/TM6 wing disc. (N) A uasngn1, dppGal4/TM6 wing disc. (O,P) Late stage embryos stained with 22C-10 (green) and anti-NGN1 (red). (O) A CS embryo. (P) A daGal4/uasngn1 embryo. (Q) A uasngn1;Gal4-7,ato1/ato1 L3 eye disc stained with anti-SENS. (Inset) A uasngn1;Gal4-7,ato1/TM6 L3 eye disc stained with anti-SENS revealing the R8 cells. |
