|
Display additional annotations [+]
|
| |
Fig. 6. A-B′) Representative control and DspMO1 morphant trunk epidermis labeled with keratin (A,B; green), beta-catenin (A′,B’; pink) and merge (A′,B′). Scale bars = 25 μm. Beta-catenin labeling (pink) was used to provide a marker of the cortical region of the cell. C,D) alpha-tubulin labeling of cells from control (C) and DspMO1 morphants (D). Scale bars = 25 μm. E,F) Phalloidin labeling of F-actin from control (C) and DspMo1 morphants (D). Scale bars = 25 μm. G) Schematic of the experimental design for H-I′. H-H′) Representative control trunk epidermis labeled with E-cadherin (J), showing the fluorescein labeled MO (H′) and merge (H′). Scale bar = 25 μm. K-K′) Representative control trunk epidermis labeled with E-cadherin (I), showing the Fluorescein labeled MO (I′) and merge (I′). Scale bar = 25 μm. Abbreviations = Ecad = E-cadherin. |
|
Display additional annotations [+]
|
| |
Fig. 4. Decreased Desmoplakin results in abnormal epidermal morphology, MCC and SSC development. A-B) Histological sections of the epidermis in control (A) and DspMO1 morphants (B). Yellow arrowheads indicate space underlying the epidermis. Scale bars = 45 μm. C-C′) Representative control trunk epidermis labeled with tubulin (C), phalloidin and tubulin (C′). Scale bars = 50 μm. D-D′) Representative DspMO1 trunk epidermis labeled with tubulin (D), phalloidin and tubulin (D′). Scale bars = 50 μm. E) Quantification of the percentage of tubulin positive cells in control compared to DspMO1 morphants. * = statistical significance. F-F′) Representative control trunk epidermis labeled with tubulin (F), phalloidin and tubulin (F′). 2X magnified region of C and C’. Scale bars = 11 μm. G-G′) Representative DspMO1 trunk epidermis labeled with tubulin (G), phalloidin and tubulin (G′). 2X magnified region of D and D’. Scale bar = 11 μm. H) Quantification of the surface area of tubulin positive cells in control compared to DspMO1 morphants. * = statistical significance. I-J′) Sections of two representative control trunk epidermis labeled with tubulin (I,J), phalloidin and tubulin (I′,J′). Scale bars = 22 μm. K-L′) Sections of two representative DspMO1 trunk epidermis labeled with tubulin (J), phalloidin and tubulin (G′). Scale bar = 22 μm. M-M′) Representative control trunk epidermis labeled with PNA (M), PNA and E-cadherin (M′). Scale bar = 50 μm. N-N′) Representative DspMO1 trunk epidermis labeled with PNA (N), PNA and E-cadherin (N). Scale bar = 50 μm. O) Quantification of the percentage of PNA positive cells in control compared to DspMO1 morphants. Blue line represents the mean. * = statistical significance. Abbreviations; PNA-peanut agglutinin, MCC = multiciliated cell, SSC = small secretory cell, OE = outer ectoderm, IE = inner ectoderm. |
|
Display additional annotations [+]
|
| |
Fig. 7. DP-NTP expression in X. laevis embryos. A) Schematic of the major domains of desmoplakin in wildtype (WT) and DP-NTP construct. B) Desmoplakin labeled with an antibody B′) DP-NTP-GFP expression B′) Merge of B and B’. C,D) Lateral views of representative control (C) and DP-NTP expressing embryos. E-E′) Trunk epidermis of a representative control embryo, labeled with tubulin (pink, E) and phalloidin (green) merged with tubulin (E′). F-F′) Trunk epidermis of a representative DP-NTP expressing embryo, labeled with tubulin (pink, F) and phalloidin (green) merged with tubulin (F′). G,H). Keratin localization in the trunk epidermis of a representative control (G) DP-NTP expressing embryo (H). Scale bars = 25 μm. |
|
|
| |
Supplemental Figure 5: Decreased Dsp specifically in the trunk caused a reduction in tubulin positive cells. A) Schematic showing the blastomere injected with MO to target the trunk epidermis. B) Trunk epidermis of a representative embryo with CMO, labeled with tubulin (pink) and showing fluorescent tagged morpholino (green). C) Trunk epidermis of a representative embryo with DspMO1, labeled with tubulin (pink) and showing region lacking fluorescent tagged morpholino (green). Scale bar= D) Trunk epidermis of a representative embryo with DspMO1, labeled with tubulin (pink) and showing fluorescent tagged morpholino (green). |
