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Figure 2. Atrial septum morphology at embryonic stage 46. The images are of 20 compiled confocal sections taken at 8-micron intervals. Embryos were fixed in Dent's fixative and used for whole-mount immunostaining. The primary antibody used was anti-cardiac troponin T (CT3) monoclonal antibody followed by incubation with anti-mouse Alexa 568 conjugate. Arrowheads point to the atrial septum and the atrioventricular canal is marked with an asterisk. A–D: A normal septum seen in a heart from a water injected control (A), a normal septum seen in a heart from an embryo injected with the XNkx2-5 full length mRNA (B), thin septum seen in a heart from an embryo injected with XNkx2-5 His156ter (C), and a frayed and deviated septum seen in the heart of an embryo injected with XNkx2-5 Gln185ter (D). E,F: Histological sections of hearts from a buffer-injected embryo (E) or an embryo injected with the XNkx2-5 His156ter mRNA (F) allow an alternative comparison of normal and frayed septum. In panels E and F, atrial nuclei were pseudocolored red using Adobe Photoshop to provide a clear view of septal structure.Download figure to PowerPoint |
