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Fig. S1. sfrp1 is not required for regulating cell proliferation. (A,C) RNA in situ sections at stage 24 show nkx2-5 expression highlighting cardiac progenitor cells. (E,G) RNA in situ sections at stage 32 show tnni3 expression within the myocardium of the differentiated heart. Note some expression remaining after loss of sfrp1 (C), whereas tnni3 expression after loss of sfrp1 is clearly confined due to the reduction in the amount of differentiated heart muscle (G). (B,D,F,H) Immunofluorescence staining of PCNA protein highlighting proliferating cells throughout the same sections as A,C,E,G. Note there is no obvious reduction in the amount of PCNA at stage 24 or at stage 32, indicating that proliferation in the heart was not the cause for loss of heart muscle in G. Scale bars: 200 μm. |
