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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
foxa2
|
|
laevis
|
NF stage 20
|
endoderm
,
foregut
,
posterior neural tube
,
floor plate
,
neural tube
|
hnf4a
|
|
laevis
|
NF stage 20
|
endoderm
|
hnf1b
|
|
laevis
|
NF stage 20
|
circumblastoporal collar
,
intermediate mesoderm
,
endoderm
,
gastrocoel roof plate
,
liver diverticulum
|
hhex
|
|
laevis
|
NF stage 20
|
endoderm
|
nkx2-5
|
|
laevis
|
NF stage 20
|
endoderm
,
foregut
,
heart primordium
|
|
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Supplementary Figure 1. Sfrp5 is required for foregut gene expression. (A-C) Gastrula stage hhex expression is unchanged by sfrp5-MO or sfrp5 RNA injection. (D-N) In situ hybridization to bisected stage 20 embryos with the indicated probes. (0 -Q) Cardiac troponin in situ hybridization to stage 35. |
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Display additional annotations [+]
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Supplementary Figure 57. Wnt11-MO control experiments. (A) Injection of the wnt11-MO (50 ng) into the dorsal midline at the 4-8-cell stage results in gastrulation defects as previously described (Tada and Smith 2000). (B) In situ hybridization with a cardiac-troponin probe at stage 35. Oorsallateral injection of the wnt11-MO (25ng) into the 4-cell stage often results in reduced cardiac differentiation (Pandur et al. , 2002). When the wnt11-MO was injected into the 01 cells at the 32-cell stage, the cardiac progenitors differentiated but fail to fuse at the midline as previously described (Garriock et al. , 2005). (C) In situ hybridization to bisected gastrula embryos with hhex shows that axial patterning and the organizer were unaffected by injection of the wnt11-MO into 01 cells at the 32-cell stage. |