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Fig.3. Spib morpholino affects myeloid cell production and also cardiac muscle morphogenesis. (A–J) Stage 31 tadpoles injected with Xtspib-e1i1MO into dorsal blastomeres (A–F), 40 ng dose, or control ventral blastomeres (G and H), or non-injected sibling (I and J), hybridized with probes for mpo and mlc2 (myocardium). Two tadpoles with severe macrophage deficit are shown (A–D) and one with a milder reduction (E and F). Myocardial phenotype severity appears linked to the number of functional macrophages produced. Anterior is to left in lateral views and ventral view of heart-forming regions. (K and L) Transverse section through the myocardium of the mild phenotype tadpole (E, F, K) compared with non-injected control (I, J, L), Nuclear-Fast Red counterstained (NFR). Section plane indicated (Sp). Scale bar = 100 μm. En, endoderm; Mc, myocardium; Ec, endocardium. (M–T) Stage 31 tadpoles injected with the spib morpholino, 40 ng dose and with same sequence of blastomere injections presented. Tadpoles hybridized to mpo and tnni3 (myocardium). One tadpole is shown with severe macrophage deficit (M and N) and one with a milder reduction (O and P). |