|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
tnni2
|
|
laevis
|
NF stage 22
|
paraxial mesoderm
|
tnni2
|
|
laevis
|
NF stage 29 and 30
|
presomitic mesoderm
,
somite
,
skeletal muscle
,
eye
,
paraxial mesoderm
,
[+]
|
tnni2
|
|
laevis
|
NF stage 35 and 36
|
presomitic mesoderm
,
skeletal muscle
,
head
,
tail
,
hypaxial muscle
,
[+]
|
tnni1.2
|
|
laevis
|
NF stage 22
|
somite
,
paraxial mesoderm
|
tnni1.2
|
|
laevis
|
NF stage 29 and 30
|
presomitic mesoderm
,
somite
,
skeletal muscle
,
paraxial mesoderm
,
tail
|
tnni1.2
|
|
laevis
|
NF stage 35 and 36
|
presomitic mesoderm
,
somite
,
skeletal muscle
,
head
,
tail
,
[+]
|
|
|
Fig. 2. Expression of Xenopus troponin I isoforms during various stages of development. (A) Whole-mount in situ hybridizations using antisense riboprobes for the troponin I fast (A–D), slow (E–H) and cardiac (I,J) mRNA isoforms during Xenopus embryogenesis (A–D and E–H represent Xenopus developmental stages 15, 22, 30 and 36 (Nieuwkoop and Faber, 1967)). Black arrows indicate the location of the hypaxial muscles while white arrows denote the location of XTnIc expression in the heart of Stage 28 (I) and Stage 30 (J) embryos. (B) RT–PCR analysis of the TnI isoforms in RNA isolated from the (A) tadpole heart, (B) tadpole tail, (C) frog heart and (D) frog limb muscles. Primer pairs specific for XTnIf (f), XTnIs (s) and XTnIc (c) were used to amplify DNA fragments of the expected sizes (f=363 bp; s=505 bp; c=874 bp). Lane M shows standard DNA size markers (1 Kb Plus DNA Ladder; Invitrogen Life Technologies). |