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tnni3xenopus   

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Experiment details for tnni3

The slow isoform of Xenopus troponin I is expressed in developing skeletal muscle but not in the heart.

The slow isoform of Xenopus troponin I is expressed in developing skeletal muscle but not in the heart.

Gene Clone Species Stages Anatomy
tnni3.L laevis NF stage 29 and 30 heart , cardiac mesoderm

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  Fig. 2. Expression of Xenopus troponin I isoforms during various stages of development. (A) Whole-mount in situ hybridizations using antisense riboprobes for the troponin I fast (A–D), slow (E–H) and cardiac (I,J) mRNA isoforms during Xenopus embryogenesis (A–D and E–H represent Xenopus developmental stages 15, 22, 30 and 36 (Nieuwkoop and Faber, 1967)). Black arrows indicate the location of the hypaxial muscles while white arrows denote the location of XTnIc expression in the heart of Stage 28 (I) and Stage 30 (J) embryos. (B) RT–PCR analysis of the TnI isoforms in RNA isolated from the (A) tadpole heart, (B) tadpole tail, (C) frog heart and (D) frog limb muscles. Primer pairs specific for XTnIf (f), XTnIs (s) and XTnIc (c) were used to amplify DNA fragments of the expected sizes (f=363 bp; s=505 bp; c=874 bp). Lane M shows standard DNA size markers (1 Kb Plus DNA Ladder; Invitrogen Life Technologies).