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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
tcp1	xenopus		optic field [+] choroid fissure,eye,eye primordium,iris,optic nerve,optic stalk,optic vesicle,bipolar neuron,retinal ganglion cell layer,retinal stem cell,retinal neural layer,peripheral retina,retinal progenitor cell region,lens fiber cell mass,cornea,ciliary body,iris,pupil,choroid,sclera,horizontal cell,retinal inner plexiform layer,optic fiber layer,ciliary marginal zone,central retina,optic nerve,retina,retinal stem cell region,lens transitional zone,pigment layer,inner limiting membrane,retinal outer nuclear layer,choroid fissure,retinal ganglion cell,retinal pigmented epithelium,lens epithelium,retinal outer plexiform layer,retinal inner nuclear layer,lens placode,Mueller cell,conjunctiva,globe,optic disc,retinal progenitor cell,lens,outer segment disk,amacrine cell,outer limiting membrane,lower eyelid,outer segment cilium,photoreceptor layer,eye photoreceptor cell,retinal layer,glial endfoot

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Experiment details for tcp1

Marracci S et al. (2015) Assay

Comparative expression analysis of pfdn6a and tcp1α during Xenopus development.

Gene	Clone	Species	Stages	Anatomy
tcp1.S		laevis	NF stage 13 to NF stage 19	optic field
tcp1.S		laevis	NF stage 22 to NF stage 23	eye , optic vesicle

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Figure 3. Whole mount in situ hybridization analysis of pfdn6a and tcp1a during neurulation. (A-C, F-H) show frontal views (dorsal to the top) of hybridized embryos at the indicated neurula stages; white arrowheads indicate neural crests. (D, I) Dorsal view of stage 18/19 embryos injected unilaterally with Rx1 RNA and nuclear LacZ RNA as a tracer (red staining). Arrows point at regions displaying stronger repression. (E, J) Lateral views of hybridized stage 22-23 embryos; anterior is to the left; nc: neural crests. Scale bars = 250um.

Gene	Clone	Species	Stages	Anatomy
tcp1.L		laevis	NF stage 13	eye primordium

tcp1 (t-complex 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 13, anterior view, dorsal up.

Gene	Clone	Species	Stages	Anatomy
tcp1.S		laevis	NF stage 42	retinal progenitor cell region , eye

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Figure 5. Cryosections of stage 42 embryos hybridized with pfdn6a (A-E) and tcp1 probes (F-J). The red arrow in (F) denotes absence of tcp1 signal in a dorsal region of prosencephalon. Black arrowheads in (A, F) indicate a region located above the pharynx, labeled with pfdn6a and tcp1, respectively. The asterisks in (B, C, G, H) indicate white matter at the level of diencephalon (B, G) and rhomboencephalon (C, H). Black arrowheads in (B, G) indicate the Jacobsons organ. The white arrowhead in (I) points to the outer plexiform layer of retina. The black arrowheads in (J) indicate facial muscles. cmz: ciliary marginal zone; ga: ganglia; le: lens; op: olfactory pits; ph: pharynx; pr: prosencephalon; rh: rhomboencephalon; vp: velar plate. Scale bars in (A-C, E, F-H, J)= 100u m; in (D, I)= 50 um.