|
Fig. 2. Manipulating the expression of Bα and Bδ in Xenopus embryos has opposing effects on Activin/Nodal target gene expression. (A) In situ hybridisation of gastrula-stage embryos injected with either a morpholino control (MoC) or a specific morpholino against Xenopus Bα (MoBα) or Bδ (MoBδ), or with Flag-tagged mouse Bδ mRNA (Bδ) at the one-cell stage. The probes used were against gsc or Xbra. Staining was visualised with BM purple. There is increased staining for both Xbra and Gsc in Bδ morphants, and decreased staining in Bα morphants and embryos overexpressing Bδ. (B) Analysis of gene expression by q-PCR. Total RNA was isolated from stage 10.5 embryos that had been injected at the one-cell stage with either morpholino control, or morpholinos against Bα or Bδ. Expression levels are normalised to ornithine decarboxylase (ODC). |