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Experiment details for tbxt

Keenan ID et al. (2006) Assay

FGF signal transduction and the regulation of Cdx gene expression.

Gene Clone Species Stages Anatomy
tbxt.S laevis NF stage 11 mesoderm , marginal zone

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  Fig. 1. Inhibition of Cdx gene expression with a dominant negative FGF receptor. Panel A shows whole-mount in situ hybridization analysis for Cdx1, Cdx2 and Cdx4 expression at gastrula stage 11 in control embryos and embryos injected with 4 ng dnFGFR1 mRNA. Panel B is an RNAase protection analysis (RPA) showing the expression of Cdx genes from mid blastula to mid gastrula stages, in control embryos and embryos injected with 4 ng dnFGFR1 mRNA. Ubiquitously expressed ornithine decarboxylase (ODC) was included as a loading control. Panel C is an RPA showing the expression of Cdx genes in control embryos and embryos injected with 4 ng dnFGFR1 or dnFGFR4 mRNA at gastrula stage 10.5. Panel D shows whole-mount in situ hybridization analysis for Brachyury (Bra), Snail (Sna) and FGF8 at gastrula stage 11 in control embryos and embryos injected with 4 ng dnFGFR1.