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Fig. 1. Inhibition of Cdx gene expression with a dominant negative FGF receptor. Panel A shows whole-mount in situ hybridization analysis for Cdx1, Cdx2 and Cdx4 expression at gastrula stage 11 in control embryos and embryos injected with 4 ng dnFGFR1 mRNA. Panel B is an RNAase protection analysis (RPA) showing the expression of Cdx genes from mid blastula to mid gastrula stages, in control embryos and embryos injected with 4 ng dnFGFR1 mRNA. Ubiquitously expressed ornithine decarboxylase (ODC) was included as a loading control. Panel C is an RPA showing the expression of Cdx genes in control embryos and embryos injected with 4 ng dnFGFR1 or dnFGFR4 mRNA at gastrula stage 10.5. Panel D shows whole-mount in situ hybridization analysis for Brachyury (Bra), Snail (Sna) and FGF8 at gastrula stage 11 in control embryos and embryos injected with 4 ng dnFGFR1. |