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Fig. 5. Mesodermal gene expression is dependent on FoxD3 function. (A-F) Control. At the four-cell stage, each blastomere was injected in the marginal zone with 500 pg of VP16-FoxD3 RNA (G-L), 25 ng of FoxD3MO (M-R), or 25 ng of mismatch MO (S-X). At the early gastrula stage (stage 10.25), embryos were analyzed by in situ hybridization for the expression of the indicated genes. The results shown are representative of three independent experiments (n=12-18 embryos per sample in each experiment). Vegetal views are shown for Brachyury, Chordin, Xwnt8, Mixer and Opl, animal views are shown for Dlx3, and dorsal is up for all panels. |
