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Experiment details for tbxt

Khokha MK et al. (2005) Assay

Depletion of three BMP antagonists from Spemann's organizer leads to a catastrophic loss of dorsal structures.

Gene Clone Species Stages Anatomy
tbxt tropicalis NF stage 10.5 mesoderm , circumblastoporal collar

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  Figure 6. Spemann’s Organizer Is Specified in FCN MorphantsAll panels are vegetal views of early gastrula embryos (st 10.5 [A–T]) or midgastrula (st 11 [U–X]) with dorsal to the right in embryos where dorsal can be distinguished. Expression of multiple mesodermal markers (brachyury [xbra] [A–D], goosecoid [gsc] [E–H], xnr3 [I–L], siamois [M–P], vent2 [Q–T], and myf5 [U–X]) are shown in uninjected sibling embryos (A, C, E, G, I, K, M, O, Q, S, U, and W) and FCN triple morphants (B, F, J, N, R, and V) as well as β-catenin morphants (D, H, L, P, T, and X) for comparison.