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Figure 2. gpx3 knockdown perturbed Wnt, Notch, and FGF signaling gene expression but did not affect the early tailbud gene expression. (A,B) gpx3 MO was injected into the ventral regions of 4-cell stage embryos, and RT-PCR was used to determine wnt3a, wnt5a, wnt5b, notch1, fgf8, and Xtbx6 gene expression after removing the anterior part of embryos. wnt3a, wnt5b, notch1, and fgf8 expressions were significantly reduced in gpx3 MO-injected embryos. * p < 0.05; ** p < 0.001; *** p < 0.0001; ns, not significant. (C) WISH analysis of gpx3 morphant embryos using Xbra and Xnot2 (chordoneural hinge and posterior wall markers). gpx3 knockdown did not affect the early tailbud gene expression. (D) RT-PCR of Xbra and Xnot2 relative expression revealed no significant differences between the control and gpx3 MO-injected embryos. |
