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Fig. 3. Overexpression of FoxO1, FoxO4, FoxO6 and daf-16 interferes with early Xenopus development. (A) Whole mount in situ hybridization of uninjected control embryos and FoxO RNA injected embryos for Xbra, Sox17α, Sox2 at stage 11 (vegetal view), Xema, stage 11 (animal view), and Chordin at stage 15 (dorsal view). RNAs were bilaterally injected at 2-cell stage with 500 pg/blastomere (FoxO1), 400 pg/blastomere (FoxO4), 200 pg/blastomere (FoxO6) and 400 pg/blastomere (daf-16). The right column shows the phenotypes of unilaterally FoxO RNA injected embryos at stage 18, dorsal view. (B) Real time RT-PCR in gain of function embryos. Injected embryos were collected at stage 11.5. RT-PCR was performed for indicated genes. Percentages of phenotypes are given in supplementary Table S2. |
