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Figure 5. Expression pattern of Tbx20 in Xenopus tropicalis.The expression pattern of Tbx20 detected by in situ hybridization between late neurula and early tadpole stages is shown (embryos uncleared). a-o: Stages are as follows: stage 20 (a-c), stage 25 (d,e), stage 29/30 (f), stage 33 (g-i), stage 36 (j-l), and stage 40 (m-o). Ventral views are shown in i, l, and o. Except for the anterior view in a, embryos are oriented with anterior to the left. a, atrium; cg, cement gland; hb, hindbrain; hp, heart primordium; ht, heart tube; sv, sinus venosus; v, ventricle. |
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Figure 6. Cardiac expression of Tbx2, Tbx5, and Tbx20. The in situ hybridization patterns of Tbx2, Tbx5, and Tbx20 in the forming cardiac chambers were examined in transverse sections through the tadpole heart after looping. a: Tbx2 expression was seen in the myocardium of both the atrial (am) and ventricular (vm) regions of the looped heart at stage 36. b: Expression of Tbx5 was restricted primarily to the developing ventricular myocardium at stage 38. c: In contrast to Tbx5, high levels of Tbx20 expression were seen in the atrial region but not in the ventricle (stage 40). Original magnification, 00. |