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Fig. 6. Xisl-1 is required for cardiac marker gene expression. 10 ng islMO were injected unilaterally into the dorsal marginal zone of 8-cell stage embryos. At st. 28 these embryos were analysed for cardiac gene expression by whole mount in situ hybridization. Xisl-1 loss-of-function results in a reduction or even loss of expression of the early cardiac gene Tbx20, as well as of the differentiation marker genes Troponin Ic (TnIc) and cardiac-α-actin on the injected side (arrows). The specificity of the morpholino effect was verified by coinjection of RNA transcribed from the δisl-1 deletion construct, whose translation is not inhibited by the islMO. Coinjection of δisl-1 RNA and islMO partially restored the expression of the investigated marker genes. |
