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Fig. 9. gli2 is required for the proper formation of NC derivatives. (A, H, K) Schematic representation of the experiments performed. (B) Diagram indicating the different components of the craniofacial cartilages. (C–F, I–M) Craniofacial cartilages preparation of stage-45 tadpoles. Alcian blue staining, ventral views of embryos, anterior side is at the top. Injected side is indicated by arrowhead. (C) GANT61 injection at stage 3 produced a reduction in craniofacial cartilages, with a more prominent phenotype in the ceratobranchial cartilage. (D) The gli2MO-injected side of embryos (arrowhead) presents a marked reduction in Meckel's, ceratohyal and ceratobranchial cartilages. (E) Rescue of cartilage morphology after coinjection of CRgli2 along with gli2MO. (F) CoMO-injected embryos showed no effect on the treated side. (G) Quantification of phenotypes shown in C–F. (H–N) Grafting of GANT61-soaked beads at two different time frames (st. 12.5 to 45 and 17 to 45) produced a consistent reduction in craniofacial cartilages. (O–Q) Lateral view of a gli2MO injected embryo. On the injected side there is a reduction in the number of melanocytes in the zone of the future gut. Quantification is shown in Fig. 9Q. (R–T) tbx2 expression shows a reduction in RB neurons and cranial ganglia primordia on the gli2MO injected side. Pr, profoundal placode; L, lens placode; Ll, lateral line placode; Tp, trigeminal placode; RB, Rohon-Beard sensory neurons.
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