| |
Figure 6. eg5 is required for the formation of neural crest derivatives formation. A–C: Craniofacial cartilages preparations of stage-46 tadpoles, Alcian blue staining. Ventral view, anterior side is at the top. Injected side is indicated by an arrowhead. A: Control morpholino CoMO-injected embryos show no effects on the treated side. B: eg5MO injected side of embryos (arrowhead) present a marked reduction in Meckel's, ceratohyal and ceratobranchial cartilages. C: The coinjection of eg5MO and Xenopus tropicalis eg5 mRNA rescues normal cartilages morphology. D: Schematic representation of eg5MO effects on Xenopus head cartilages. E, E', E'': eg5MO-injected embryos show altered Rohon-Beard sensory neurons and cranial ganglia precursors derivatives. Notice that tbx2 expression pattern was altered in the injected side of embryos. M, Meckel's cartilage; CH, ceratohyal cartilage; CB, ceratobranchial cartilage; Ir, infrarostral cartilage; BH, basihyal cartilage; Pr, profoundal placode; L, lens placode; Ll, lateral line placode; V, trigeminal placode; Cg, cement gland; RB, Rohon-Beard sensory neurons. A-D, black arrowhead, injected side. E, E', white arrowhead, injected side; black arrow, cranial ganglia precursors; black arrowhead, lens placode; Otp, otic placode.Download figure to PowerPoint |
